The Center for human immunology provides technologies that allows single cell profiling & sequencing as well as imaging flow cytometry and live cell imaging. Some technologies are located in a BSL2+ facility.
TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]
Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below: Technology Autonomous Assisted Training* Academic / Industry Cytometers & […]
Seahorse Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- […]
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]
BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]
nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]
Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]
BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]
Bioanalyzer Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.
Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
Charlie Brown 5 lasers (355nm, 405nm, 488nm, 561nm and 640nm), with “index sorting” (software FACSDiva 7) Configuration Charlie Brown Build your panel with the instrument configuration: FACS ARIA II Charlie Brown This FACSAria sorter is located […]
Equipped with four laser lines (355nm, 405nm, 488nm and 640nm), 17 fluorescent detectors and an HTS unit for high-throughput screening in plates
ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
intuitive web-based applications for quickly analyzing and accurately interpreting the biological meaning in real-time PCR, sequencing or microarray data.
software developed for the analysis of ImageStreamX data; it evaluates over 35 features for each image and over 200 features per cell and integrates population statistics and imagery – every dot on a scatter […]
2019Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis, Nat Commun 2019 04;10(1):1929.
2017Role of the IL-12/IL-35 balance in patients with Sjögren syndrome, J. Allergy Clin. Immunol. 2018 Jul;142(1):258-268.e5.
2017Single-cell gene expression using multiplex RT-qPCR to characterize heterogeneity of rare lymphoid populations, J. Vis. Exp. JoVE 2017 Jan 19; (119).
2016Standardized Whole-Blood Transcriptional Profiling Enables the Deconvolution of Complex Induced Immune Responses, DOI: http://dx.doi.org/10.1016/j.celrep.2016.08.011 show.
2016Inhibition of DPP4 activity in humans establishes its in vivo role in CXCL10 post-translational modification: prospective placebo-controlled clinical studies, EMBO Mol Med 2016 Jun;8(6):679-83.
2016Protein biomarkers discriminate Leishmania major-infected and non-infected individuals in areas endemic for cutaneous leishmaniasis, BMC Infect. Dis. 2016;16:138.
2014Baseline sensitivity of T cells to alpha-IFN correlates with sustained virological response to IFN-based triple therapy in HCV infection, J. Viral Hepat. 2015 Jun;22(6):524-34.
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