The Center for human immunology provides technologies that allows single cell profiling & sequencing as well as imaging flow cytometry and live cell imaging. Some technologies are located in a BSL2+ facility.
TRAINING The team provides training for the technologies and data-analysis. The trainings are scheduled on a monthly basis. Theoretical and hands-on training sessions are designed for basic and advanced autonomous users. All trainings are […]
Autonomous and assisted use of CB UTechS technologies, as well as training sessions for technologies and software are associated to a cost, as summarized below: Technology Autonomous Assisted Training* Academic / Industry Cytometers & […]
Seahorse Simultaneous analysis of mitochondrial respiration and glycolysis in 96-well plates. With four programmable ports for the addition of liquid media during active assay measurements, it is ideal for the study of chemical-, adjuvant- […]
Quantitative live cell imaging in a standard incubator using any traditional culture plate/dish. IncuCyte supports HD phase-contrast, green fluorescence and red fluorescence at 20x magnification. With its remote control and access to the real-time […]
BioPlex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different analytes in the […]
Ion semiconductor sequencing for exome, genome & transcriptome high throughput sequencing at reduced cost and complexity. The sequencing run times of <4h and 200bp reads of up to 10Gb (Ion Proton) and <7h and 400 […]
nCounter FLEX NanoString A digital color-coded barcode technology for direct multiplexed measurement of gene expression. It allows up to 800 mRNA, miRNA or DNA targets to be simultaneously profiled based on molecular “barcodes” and […]
Fluidigm C1 Microfluidic-based technology that enables single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell […]
BioMark Fluidigm The microfluidics-based technology Biomark, is an automated, high-performance PCR system that processes tens to hundreds of samples in parallel at nanoliter volumes for gene expression, genotyping, copy number variation analysis and digital […]
Bioanalyzer Chip-based capillary electrophoresis machine for quantification and quality control of DNA, RNA, & proteins. It allows RNA and DNA quality control measurements before downstream experiments.
Qubit 2.0 Sensitive quantification of DNA, RNA & proteins using fluorescent dyes. Qubit allows distinguishing between DNA, RNA or free nucleotides and the measurement is not affected by the presence of co-purified contaminants.
ImageStreamX Integrates flow cytometry (3 lasers) and microscopy (60x magnification) to simultaneously produce 12 images of each cell directly in flow, at rates exceeding 15 000 cells/min. Enables quantification of cell shape changes, internalization, apoptosis, […]
intuitive web-based applications for quickly analyzing and accurately interpreting the biological meaning in real-time PCR, sequencing or microarray data.
software developed for the analysis of ImageStreamX data; it evaluates over 35 features for each image and over 200 features per cell and integrates population statistics and imagery – every dot on a scatter […]
2019Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis, Nat Commun 2019 04;10(1):1929.
2017Role of the IL-12/IL-35 balance in patients with Sjögren syndrome, J. Allergy Clin. Immunol. 2018 Jul;142(1):258-268.e5.
2017Single-cell gene expression using multiplex RT-qPCR to characterize heterogeneity of rare lymphoid populations, J. Vis. Exp. JoVE 2017 Jan 19; (119).
2016Standardized Whole-Blood Transcriptional Profiling Enables the Deconvolution of Complex Induced Immune Responses, DOI: http://dx.doi.org/10.1016/j.celrep.2016.08.011 show.
2016Inhibition of DPP4 activity in humans establishes its in vivo role in CXCL10 post-translational modification: prospective placebo-controlled clinical studies, EMBO Mol Med 2016 Jun;8(6):679-83.
2016Protein biomarkers discriminate Leishmania major-infected and non-infected individuals in areas endemic for cutaneous leishmaniasis, BMC Infect. Dis. 2016;16:138.
2014Baseline sensitivity of T cells to alpha-IFN correlates with sustained virological response to IFN-based triple therapy in HCV infection, J. Viral Hepat. 2015 Jun;22(6):524-34.
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