Externe collaboration with Genomic, Structure and Translation team of Olivier Namy
Translation fidelity is essential for the correct decoding of genetic information. However, translation fidelity is not maximal, and several genes use alternative mechanisms to regulate their expression. Genome-wide translational analyses, such as ribosome profiling, can identify many features affecting translational quality, such as ribosomal pauses, dual coding regions, translation start sites at AUG or non-AUG codons or unconventional translational decoding events, the translation of non-coding RNA and translational ambiguities.
Ribosome profiling has been widely adopted by the scientific community, because it has revolutionised the ways in which translation can be studied. It can be used for both quantitative and qualitative analyses of translation.
The Genomic, Structure and Translation Team of the Institut de Biologie de la Cellule (Université Paris-Saclay – CNRS) use ribosome profiling approach for understanding translation fidelity in yeast and human cells.