Characterization of the SigmaS network by RNA sequencing and identification of SigmaS-controlled small regulatory RNAs in Salmonella

Présentation

We have used directional RNA-sequencing and complementary assays to explore the SigmaS-dependent transcriptome of Salmonella enterica serovar Typhimurium. This study confirms the large regulatory scope of SigmaS and provides insights into its physiological functions in Salmonella. Extensive regulation of genes involved in metabolism and membrane composition, and downregulation of the respiratory chain functions, were important features of the SigmaS effects on gene transcription that might confer fitness advantages to bacterial cells and/or populations under starving conditions. The SigmaS network includes small RNAs that might endow SigmaS with post-transcriptional regulatory functions.