Identification of cellular PDZ-containing Proteins targeted by the virus SARS-Cov-2 during infection (PPCOVi)
Thanks to their binding to PDZ domains, viral proteins play an important role in the replication and pathogenesis of SARS-CoV. Through cellular and in vitro high-throughput tests, this project aims to identify the cellular […]
Role of PDZ-binding motif from Flavivirus non-structural proteins in flaviviral life cycle
Arthropod-borne viruses (arboviruses) include numerous human and animal pathogens that are important global health threats. Flaviviruses are arboviruses belonging to the Flaviviridae family. They include neurotropic viruses such as West Nile (WNV), Japanese encephalitis […]
Interplay between influenza virus and the ubiquitin/proteasome system
As a major pathway of reversible protein modification in eukaryotes cells, leading to their degradation, affecting their subcellular localization, or activity, the Ubiquitin Proteasome System (UPS) is a prime target of viruses. Recent evidence […]
Multi-species mapping of influenza viruses cellular targets using high-throughput complementary strategies in a “One Health” perspective.
Despite medical progress, the recent outbreaks of viral zoonoses (e.g. MERS-CoV, Ebola virus disease) illustrate the constant threat represented by viruses in a world in motion and globalization. These epidemic events stress the need […]
Comparative interactomics of filoviridae (Task Force Ebola WP6.2)
To date, knowledge on the interaction network between filovirus proteins and the host proteome (virhostome) are still extremely small and fragmented. These studies are limited to a few proteins such as VP24, VP35 and […]
Design and optimization of new bioluminescent systems better-suited to in cellulo and in vivo conditions
The use of light from the abyss in life sciences In the last decades, bioluminescent systems based on the expression of a luciferase and the addition of a luciferin to monitor the emission […]
Optogenetic “Cre-stiching” strategy for barcode fusion genetics
we developed an optogenetic “Cre-stiching” strategy for barcode fusion genetics. This new technology is based on light-inducible Cre-recombinase complementation. The resulting optogenetic control Cre-recombinase produces a concatenated DNA, linking together genetic barcodes. These concatenated […]
High-throughput protein-protein interaction assay based on a split-luciferase
The coverage and robustness of protein-protein interactions (PPI) datasets can be markedly increased by combining orthogonal PPI assays. For this purpose we have recently developed two variations of our High-throughput Gaussia princeps Complementation Assay(HT-GPCA), […]
2018The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome, Oncotarget 2018 Mar;9(17):13102-13115.
2017Inhibition of the inflammatory response to stress by targeting interaction between PKR and its cellular activator PACT, Sci Rep. 2017 Nov 23;7(1):16129.
2015Amyloid Precursor-like Protein 2 and Sortilin Do Not Regulate the PCSK9 Convertase-mediated Low Density Lipoprotein Receptor Degradation but Interact with Each Other, J. Biol. Chem. 2015 Jul;290(30):18609-20.
2015Quantifying domain-ligand affinities and specificities by high-throughput holdup assay, Nat. Methods 2015 Aug;12(8):787-93.
2015Targeting the Two Oncogenic Functional Sites of the HPV E6 Oncoprotein with a High-Affinity Bivalent Ligand, Angew. Chem. Int. Ed. Engl. 2015 Jun;54(27):7958-62.
2015Widespread macromolecular interaction perturbations in human genetic disorders, Cell 2015 Apr;161(3):647-60.
2015A field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions, Methods Mol. Biol. 2015;1282:213-29.
2014Microtubule-associated proteins 1 (MAP1) promote human immunodeficiency virus type I (HIV-1) intracytoplasmic routing to the nucleus, J. Biol. Chem. 2015 Feb;290(8):4631-46.
2014Systematic screening reveals a role for BRCA1 in the response to transcription-associated DNA damage, Genes Dev. 2014 Sep;28(17):1957-75.
2014Recruitment of RED-SMU1 complex by Influenza A Virus RNA polymerase to control Viral mRNA splicing, PLoS Pathog. 2014 Jun;10(6):e1004164.
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