Jacomine Krijnse Locker is a group-leader and heads the Ultrapole (platform of ultrastructural microscopy) at the Institut Pasteur in Paris. She obtained her PhD in Utrecht, the Netherlands, in 1994 on cellular aspects of coronaviruses.
Embeding methods for salivary glands of mosquitos.
With the group of Anna-Bella Failloux we established a method to embed salivary glands of mosquitos infected with Chikungunya virus to study its replicative cycle in situ (Vega-Rua et al., 2016; viruses 7: pp. […]
FIB-SEM to study anterograde/retrograde transport in the flagellum of trypanosomes.
With the group of Philippe Bastin we have a long-standing collaboration to study the structure of protein complexes (or “trains”) that are moved by anterograde and retrograde transport in the flagellum of trypanosomes. Towards […]
Correlative approaches to study phagosomal membrane rupture.
The group of Jost Enninga studies the escape of intracellular bacteria from phagosomes using advanced imaging techniques. The aim is to study the process of phagosomal membrane rupture that mediates bacterial release into the […]
TNTs characterization by CryoEM
The group of Chiara Zurzolo studies the function, structure and formation of tunneling nano tubes (TNTs) that connect neuronal cells (Delage et al., 2016; Sci Rep 6: 39632). We provide EM support to characterize […]
The research group of the UBI studies membrane assembly of viruses, focusing predominantly on large DNA viruses. Using tools collected over the years, we use virus-infected cells to develop advanced EM-embedding and imaging methods that can be applied by the users of our platform. We put a particular emphasis on the development on two aspects in EM. The development of high-resolution cryo-EM methods to determine the structure of viral membrane-associated proteins in vitro and in situ. We also wish to broaden our portfolio of correlated- light and electron microscopy methods. For both we develop workflows that involve sample preparation, semi-automated imaging and image processing. We particularly welcome the input of autonomous users that can complement our efforts along their biological questions.
Chlanda, P., Carbajal, M.A., Cyrklaff, M., Griffiths, G., and Krijnse Locker, J. (2009). Membrane rupture generates single open membrane sheets during vaccinia virus assembly. Cell Host & Microbe 6, 81-90.
Chlanda, P., and Krijnse Locker, J. (2017). The sleeping beauty kissed awake: new methods in electron microscopy to study cellular membranes. Biochem J 474, 1041-1053.
Krijnse Locker, J., Chlanda, P., Sachenheimer, T., and Brügger, B. (2013). poxvirus membrane biogenesis: rupture not disruption. Cell Microb 15, 190-199.
Krijnse Locker, J., and Schmid, S.L. (2013). Integrated electron microscopy: super-duper resolution. PLoS Biol 11 ppe1001639.
Miller, S., and Krijnse-Locker, J. (2008). Modification of intracellular membrane structures for virus replication. Nat Rev Microbiol 6, 363-374.
Muller, B., and Krijnse-Locker, J. (2014). Imaging of HIV assembly and release. Methods Mol Biol 1087, 167-184.
Suarez, C., Hoppe, S., Penard, E., Walther, P., and Krijnse Locker, J. (2017). Vaccinia Virus A11 Is Required for Membrane Rupture and Viral Membrane Assembly. Cellular microbiology.
Welsch, S., Keppler, O.T., Habermann, A., Allespach, I., Krijnse Locker, J., and Krausslich, H.-G. (2007). The primary site of HIV-1 budding in infected primary macrophages is the plasma membrane. . PLOS pathogen 3, 1-11.
Welsch, S., Miller, S., Romero-Brey, I., Merz, A., Bleck, C.K.E., Walther, P., Fuller, S.D., Antony, C., Krijnse-Locker, J., and Bartenschlager, R. (2009). Composition and three-dimensional architecture of the dengue virus replication and assembly sites. Cell Host & Microbe 5, 365-375.
2019Complex membrane remodeling during virion assembly of the 30,000 years-old Mollivirus sibericum, J. Virol. 2019 Apr;.
2019Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells, Nat Commun 2019 01;10(1):342.
2018Entry and Disassembly of Large DNA Viruses: Electron Microscopy Leads the Way, J. Mol. Biol. 2018 Jun;430(12):1714-1724.
2017MybA, a transcription factor involved in conidiation and conidial viability of the human pathogen Aspergillus fumigatus, Mol. Microbiol. 2017 Sep;105(6):880-900.
2017The sleeping beauty kissed awake: new methods in electron microscopy to study cellular membranes, Biochem. J. 2017 03;474(6):1041-1053.
2015Chikungunya Virus Replication in Salivary Glands of the Mosquito Aedes albopictus, Viruses 2015 Nov;7(11):5902-7.