The main goal of the project is to study the impact of bacterial infection on blood vessel function using an in-house designed microfluidic device. In collaboration with the Institut Pasteur Biomaterials and Microfluidics core facility (BMcf), the host lab has developed a vessel-on-a chip device mimicking 3D cellular architecture of blood vessels. These engineered 3D vessels are produced through in situ patterning within 3D cell-laden hydrogels using laser ablation.
The key biological question of the project will be to understand how bacteria alter vessel physiology in the context of sepsis. The Gram-negative bacterium Neisseria meningitidis will be used as a model. Spinning disk confocal microscopy will be the tool of choice to image the impact of infection on endothelial cells and their function. This project thus contains a strong component of cell biology and different imaging approaches
This research will take place in a young and dynamic group with a strong background in biochemistry, microbiology, cell biology and animal models of infection. The Institut Pasteur microfluidics core facility will provide technical support and contribute with the rest of the campus to providing a stimulating environment for the project.
For this project, we are looking for a motivated young scientist with interest in multidisciplinary approaches including organ-on-a-chip and 3D cell culture systems, cell biology, infectious diseases and fluorescence microscopy. Prior notions in one or several of these fields would be a strong plus. This Master 2 project can open the way to a PhD.
Unité “Pathogenèse des infections vasculaires”
28 Rue du Dr Roux
01 44 38 93 83