(Humboldt-Universität zu Berlin Institut für Biologie)
GENOMES AND GENETICS DEPARTEMENTAL SEMINAR
ABSTRACT: Dnmt2 is a tRNA methyltransferase that methylates cytosine 38 on tRNA-Asp. In our earlier work, we found that Dnmt2 activity in the fission yeast Schizosaccharomyces pombe is strongly stimulated by prior queuosine (Q) modification of the tRNA at the wobble position (Q34). Q is synthesized by eubacteria and salvaged by eukaryotes to replace G34 in tRNAs. We show by ribosome profiling in S. pombe how Q and Dnmt2 globally affect translation, and we find effects of Q on the suppression of erroneous codon reading. We furthermore show that Q stimulates tRNA-Asp methylation by Dnmt2 in mice. Our findings demonstrate an unanticipated mechanism for the regulation of translational speed and fidelity by a bacterial nutrient.
Bâtiment: Bâtiment Monod (sous-sol)
Salle: Amphithéâtre Jacques Monod
Address: Institut Pasteur, Rue du Docteur Roux, Paris, France