Kaustuv Sanyal – JNCASR, Bangalore, India
Organisms in the Cryptococcus species complex of the fungal phylum of Basiodiomycota divide by budding. Some of them are non-pathogenic but others are pathogenic which can cause diseases ranging from pneumonia-like symptoms to fungal meningitis primarily to immunosuppressed but occasionally to even healthy humans. Aneuploidy-related drug resistance is common in these organisms. In my presentation, first I will present how RNAi is involved in shaping the centromere structure and how centromere-mediated inter-chromosomal recombination might have helped transition from a tetraploar mating system in nonpathogenic species to a bipolar system in pathogenic species. Next, I will show evidence of loss of a major evolutionarily conserved kinetochore sub-complex in basiodiomycetes and an emergence of novel basiodiomycete-specific kinetochore proteins. Finally, I will present our efforts to understand how cell cortical forces regulate equal distribution of the nucleus between two daughter cells to prevent aneuploidy in this organism using a combined cell biology and systems biology approach.
Useful links related to the presentation :
Chromosome segregation in fungal pathogens: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107279/
Centromere evolution: https://www.ncbi.nlm.nih.gov/pubmed/29507212
Mating-type evolution: https://www.ncbi.nlm.nih.gov/pubmed/28800596
Developing a computation model: https://www.ncbi.nlm.nih.gov/pubmed/26310442
Cortical forces in nuclear division: https://www.ncbi.nlm.nih.gov/pubmed/30763303