The platform is mainly using Illumina Next Generation Sequencing for transcriptomics and epigenomics (mainly using RNAseq and ChiPseq approaches). It is accessible to Research Units from the Campus, the network and to external teams. The plaform has wet lab (sample quality control and processing) and dry lab (bioinformatics and statistics) activities.
It has two functioning modes: service (sequencing of libraries prepared by the users and providing of the raw data) and, most often, collaboration (in this case, the platform is involved in all the steps of the project, from the experimental design to the statistical analysis of the data). We work closely with the C3BI and DSI to provide data analysis solutions and data storage for researchers.
Submission of a project to the platform is a ONE STEP process:
Please contact us for pricing and turnaround times.
In case of multiplexing, choosing the right indexes is of paramount importance. Please look at the following : Index compatibility for sequencing before choosing your indexes.
WARNING: When bringing the samples to the platform please complete this EXCEL FILE according to your project specifications. Note that:
- sample IDs must be short, informative and without special characters such as @, #, $, +, -, etc.
- feel free to add any column if needed (date, concentration, …)
- the Excel file must be as simple as possible: avoid colors, bold characters, cell fusion…
Once completed, you have to upload the excel file onto the Bistro web site (https://biomics.pasteur.fr/projects/). You must be logged as user and select modify project. You can then upload the file as additional information.
Hereafter is an example of such a file:
Since 2008, the platform has been involved in more than 330 projects. These cover a wide range of organisms (viruses, bacteria, fungi, protozoans, mammals) and are centered on the main biological questions under study at the Pasteur Institute.
They are focused on the following themes (not exhaustive):
- Study of virulence factors
- Study of host-pathogen interactions, and host response to infection
- Study of the effect of environmental pressures (stress, immune pressure, drug pressure …) on the pathogen
- Comparison of inter-species transcriptomes
- Identification of regulatory targets (virulence, motility...)
- Developmental Biology