The platform is mainly using Illumina Next Generation Sequencing for transcriptomics and epigenomics (mainly using RNAseq and ChiPseq approaches). It is accessible to Research Units from the Campus, the network and to external teams. The plaform has wet lab (sample quality control and processing) and dry lab (bioinformatics and statistics) activities.
It has two functioning modes: service (sequencing of libraries prepared by the users and providing of the raw data) and, most often, collaboration (in this case, the platform is involved in all the steps of the project, from the experimental design to the statistical analysis of the data).
Submission of a project to the platform is a TWO STEP process:
FIRST STEP: fill the general project submission form for the Biomics pole
SECOND STEP: fill the appropriate form for the platform according to wether your project is a collaboration or a service
For service projects, a Application Form has to be downloaded pf2_formulaire-soumission-service and sent to Jean-Yves Coppée. In case of multiplexing, choosing the right indexes is of paramount importance. Please look at the following : Index compatibility for sequencing before choosing your indexes.
Since 2008, the platform has been involved in more than 330 projects. These cover a wide range of organisms (viruses, bacteria, fungi, protozoans, mammals) and are centered on the main biological questions under study at the Pasteur Institute.
They are focused on the following themes (not exhaustive):
- Study of virulence factors
- Study of host-pathogen interactions, and host response to infection
- Study of the effect of environmental pressures (stress, immune pressure, drug pressure …) on the pathogen
- Comparison of inter-species transcriptomes
- Identification of regulatory targets (virulence, motility...)
- Developmental Biology