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About

The Recombinant Proteins Platform provides high-quality recombinant proteins for structural (X-ray and NMR) or functional studies, produced from a wide range of expression systems: E. coli, Saccharomyces, Pichia pastoris, and Leishmania tarentolae. PFPR has the expertise staff and large-scale range equipment to deliver purified proteins through a standardized “pipeline”: screening of host-vector expression systems in micro-plates on automated Tecan platform, optimization of processes in 100 ml micro-fermenters, culture scale-up in large volume conventional bioreactors, chromatographic protein purifications using AKTA systems. A major goal of our platform is to implement new technological tools and diversify ours expression systems in order to provide optimal service in terms of quality and throughput. PFPR provides strains, vectors, technical supports, and ensure training for students. Our platform is open to scientific collaborations with academic institutions, and technological co-developments with industrial partners.

 

Projects

Equipments

Fermentor • Eppendorf • Bioflo® 320

The Eppendorf Bioflo® 320 fermentors are intermediate systems ideal for pilot through production applications. Applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Components : Working volume from 0.6 to 5.5 L. Rushton type impeller. 1 TMFC […]

Fermentor • New Brunswick Scientific / Eppendorf • Bioflo® 510

The New Brunswick™ Bioflo 510 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Batch or fed batch of E. coli […]

2 • Fermentor • Eppendorf • Bioflo® 310

The Eppendorf Bioflo® 310 fermentors are intermediate systems ideal for pilot through production applications. Our applications : Laboratory and pilot scale fermentation of aerobic bacteria and yeasts. Production of recombinant proteins in E. coli, M. smegmatis, S. cerevisiae, […]

Automated screening Platform • TECAN • Evo 200

The Tecan automated platform is used for rapid cultures screening in μ-24 well micro plate to select the best strain-vector couples and simultaneous evaluation of several culture parameters, as well for analytical protein purifications […]

BiopodF200 microfermenters unit • Fogale Nanotech •

The BiopodF200 (developed and patented by the PFPR) allows processes optimization for E.coli, yeast or different microorganisms in 100 ml parallel and independent micro-fermentors. This scale-down step is essential for the reliability of the […]

20-L bioreactor • Eppendorf • Bioflo 510

PFPR is equipped with conventional fermenters of 2-L up to 20-L, for cultures of E. coli or yeast in complex or labeled chemically defined media. We have developed an home-made high-density culture medium for […]

Cells distrupter • Constant Cells distrupter- Cell D •

The Constant cells distrupter, is used for automatic and continuous breaking cell suspension up to 200 ml per cycle (E.coli, or yeasts). It’s a very efficient and friendly use system that ensures a strict […]

Protein purification equipment • GE Healthcare/ AKTA pure and AKTA Express •

The AKTA pure system and AKTA Express units are used for complex or conventional protein purification protocols (affinity resins, Ions Exchange, Gel-Filtration chromatography). The PFPR purified both soluble proteins and inclusion bodies involving protein […]

Publications

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Contact

 

Phone: 01 40 61 35 98
Email: bellalou@pasteur.fr

PFPR. Fernbach building. 1st basement
25-28 Rue du Docteur Roux
75015, Paris
France