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© Institut Pasteur
Cellules de chenille lépidoptère infectées par un Baculovirus recombinant, qui exprime le gène de la protéine d'enveloppe du virus de la dengue. Cette protéine pourrait servir à l'élaboration d'un vaccin contre la dengue. Le Baculovirus est un virus qui infecte les cellules d'insectes et est l'un des virus utilisés comme vecteurs pour l'expression de gènes étrangers dans des cellules d'insecte.
Scientific Fields
Diseases
Organisms
Applications
Technique

About

This platform provides  production and purification of proteins from eukaryotic cells (mammals and insects) and the following expression systems:

  • baculovirus / insect cells
  • Drosophila cells (Schneider)
  • Mammalian cells (e.g. HEK293)
  • Large-scale Culture of hybridoma

The platform also ensures the development and adaptation of new technologies for the production and purification of proteins.

Equipments

3 • Bioreactors • GE Healthcare • Wave 20/50 EHT / Wave POD II

The disposable WAVE Bioreactor systems were designed based on a non-invasive rocking motion that provides excellent mixing and gas transfer for cell growth. WAVE bioreactor system is a reliable and intuitive cell culture device for […]

Flexstand • GE Healthcare • flexstand

FlexStand benchtop pilot scale systems is compact, sanitary hollow fiber ultrafiltration/microfiltration systems for laboratory through pilot scale applications. This cross flow system is designed for fast, efficient concentration and/or diafiltration of a wide range of biological […]

2 • Akta • GE Healthcare • Flux

ÄKTA flux is a versatile cross flow filtration system for sample concentration and diafiltration as well as cell harvest and clarification.

Akta • GE Healthcare • CrossFlow

ÄKTAcrossflow is a fully automated cross flow filtration system for process development and it is suitable for filter screening and process optimization at small-scale as well as small-scale processing.

4 • Akta • GE Healthcare • Avant / Explorer / Purifier

chromatography systems designed for preparative chromatography for fast and secure developement and scalable methods and processes.  

Publications

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