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© William Beaucardet
Reportage Laboratoire des Anticorps en Thérapie et Pathologie
Service

Private: Single Cell Resources at the Institut Pasteur

Starting Date
18
Oct 2019
Status
Ongoing
Members
1
Structures
1

About

Cell-to-cell variation affects all biological systems and processes, from stem cells and development, to immunology or cancer. Conventional population (bulk) measurements provide averaged information that does not reveal cellular individuality and masks extreme phenotypes that may occur within a given cellular population. Recent breakthroughs in single-cell omics technologies have made it possible to transpose the large-scale studies down to single cell level and to investigate the genome, epigenome, transcriptome, proteome, metabolome, and their inherent interactions with single-cell resolution.

The CB UTechS enables researchers to gain access to high-end instrument portfolio for single cell analysis. We provide expert advise on experimental design, instrument training and support to users trough dedicated professionals.

We offer an end-to-end solution from study design, to cell enrichment by FACS, to single cell isolation, to library preparation and bioinformatic data analysis.

single cell Genomics

Cytometry

sc-Proteomics

sc-Imaging

 

Technolgies

10X Chromium

Enables RNAseq of 200-10000 single cell per sample and 8 samples in parallel. Cells are partitioned into microfluidic droplets.

 

 

Different applications are available at the platform:

MARS-seq

 

Biomark
The Biomark can run microfluidic chips (Integrated Fluidic Circuits, IFC) for massively parallel QPCR of up to 96 cells, targeting up to 96 genes. The system utilises low volumes of standard gene expression nor
genotyping TaqMan or Evergreen systems. Different IFCs format are available to detect 48 genes in 48 cells, 96 genes in 96 cells and a flexible modular and reusable IFC is also available for up to 72 samples.
C1

Microfluidic-based technology that enables automated on-chip single cell preparation, RNA extraction, reverse transcription and PCR amplification using just one technology and thus reducing the variability caused by multi-platform technical errors. It supports multiple single-cell applications, allowing global transcriptome analysis, targeted gene expression, miRNA analysis and whole genome amplification for DNA sequencing.

Polaris
The Polaris system is a flexible microfluidics platform that allows to perform single-cell gene expression analysis. Under automated control, Polaris can image, select, capture, culture, and dosesingle cells individually. The Polaris instrument uses IFC (integrated fluidic circuit) to captures 48 cells varying in size from 5 to 25 microns and is capable of measuring responses to environmental changes by allowing treatment and culturing of cells for up to 24 hours.

 

Automation

Bravo platform

 

Quality control and quantification

Bioanalyzer

Tape station

Qubit

 

Access Protocol

PPMS