With challenging samples, like RNA isolated using laser capture microscopy, it is difficult to obtain 10 ng of starting material; additionally, rRNA depletion leads to sample loss, undermining robust library preparation. The new release surpasses the previously achieved lower input limit by incorporating a proprietary technology in which ribosomal cDNA is removed after creating the complete cDNA library, thereby enriching RNAs of interest—namely mRNA and non-polyadenylated RNA. The SMARTer Stranded Total RNA-Seq Kit – Pico Input Mammalian provides a unique, sensitive, and ligation-free method to generate stranded, Illumina-ready cDNA libraries from an input range of 250 pg–10 ng of total mammalian RNA in about 5 hours. The kit performs equally well with high-quality, partially degraded, or low-quality total RNA.
For more information on SMART technology, please visit www.takarabio.com This schematic shows the technologies integrated into and workflow of this product: