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  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
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  • Physician
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  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
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  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
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  • Labex Coordinator
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
18
Nov 2015
Status
Ongoing
Members
4
Structures
1

About

Illumina uses a green laser to sequence G/T and a red laser to sequence A/C. At each cycle at least one of two nucleotides for each color channel need to be read to ensure proper registration.

Access Protocol

It is important to maintain color balance for each base of the index read being sequenced, otherwise index read sequencing could fail due to registration failure. Please check the real sequences of each index in the table above to make sure each base position will have signal in both color channels for the index read:

index-compatiblility

For example, the following table illustrate possible pooling strategies. These represent only some of the acceptable combinations:

This recommendation is available for all index (Truseq, Nextera, “Home made” ….).

index-truseqsetA

An web application called checkMyIndex and available here has been developped at the Biomics Platform to help in finding compatible indexes according to some parameters (e.g. total number of samples or multiplexing rate).