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© Fabrice Chrétien with Ultrapole, colorized by Jean-Marc Panaud
Cellule souche (en jaune) de muscle squelettique partiellement recouverte par la membrane basale, migrant sur une fibre musculaire (en bleu).
Starting Date
19
Nov 2015
Status
Ongoing
Members
7
Structures
1

About

About us

Our laboratory offers state-of the art large histotechnological laboratory with a team of specialized histopathologists both from medical and veterinary education. The pathologists of our unit are available for collaborative research. Many projects are currently ongoing on animal models of infectious diseases, cancer, immunotherapy or inflammation with internal or external research teams. Individuals wishing to develop collaborative research efforts are encouraged to contact us when developing research projects. Optimal results are obtained when the pathology staff is contacted as early as possible in the conception of the in vivo part of the project; this allows us to help determine how histological approach can best support the research aim.

Range of services

A variety of pathology services can be provided to research investigators:

  • Necropsy & gross examination of animal tissues
  • Design of the protocol
  • Methodology advice on proper tissue collection
  • Choice of morphological approaches (fixation, embedding, slide preparation, slide treatments)
  • Evaluation of the slides including description, semi-quantitative scoring and morphometric analysis
  • Histopathology report with microphotographs
  • Discussion of the results
  • Recommendations for further experiments

Techniques

Our Unit includes a histology laboratory that prepares slides and a wide variety of quality, flexible histopathological and immunohistochemical stains for pathologists and investigative staff. We also develop new technical tools for animal tissue analysis:

  • Low-melting point paraffin embedding for immunolabeling of labile membrane antigens
  • Low-mel paraffin embedding for beat-galactosidase activity detection
  • Thick sections (Vibratome®)
  • Acrylic resin semi-thin sections
  • Large sections of a large organs or whole animal
  • We have access to Safety level 3 facility for frozen sectioning of infected human or animal tissues.

Terms of collaboration/authorship

Our scientific contribution is free-of-charges but collaborators will be asked to share the cost of histological techniques. Pathologists who contributed to the project must be listed as co-authors.

Access Protocol

If you wish to collaborate with us for the morphological analysis of animal tissues, please send  your request to

Laurence.Fiette@pasteur.fr

with the following information

  • Specify the name of the collaborator(s), team and head
  • Provide a short description of the project (one page)
  • Join relevant publications on the subject
  • Define the aim of the histopathological study

We will not accept tissue samples from a new collaborator without preliminary contact and scientific discussion