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© Research
Publication : Diagnostic microbiology and infectious disease

Whooping cough in South-East Romania: a 1-year study

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Diagnostic microbiology and infectious disease - 26 Nov 2013

Dinu S, Guillot S, Dragomirescu CC, Brun D, Lazăr S, Vancea G, Ionescu BM, Gherman MF, Bjerkestrand AF, Ungureanu V, Guiso N, Damian M

Link to Pubmed [PMID] – 24355701

Diagn. Microbiol. Infect. Dis. 2014 Mar;78(3):302-6

The incidence of whooping cough in Romania is substantially underestimated, and, as noted by the health authorities, this is mostly due to the lack of both awareness and biological diagnosis. We conducted a 1-year study in Bucharest in order to assess the circulation of Bordetella pertussis, the main etiological agent of whooping cough. Fifty-one subjects suspected of whooping cough were enrolled. Culture, real-time PCR, and enzyme-linked immunosorbent assay were used for laboratory diagnosis. Whooping cough patients (63%) were distributed among all age groups, and most were unvaccinated, incompletely vaccinated, or had been vaccinated more than 5 years previously. Bordetella holmesii DNA was detected in 22% of the bordetellosis cases; these patients included adults; teenagers; and, surprisingly, young children. B. pertussis isolates were similar to the clinical isolates currently circulating elsewhere in Europe. One isolate does not express pertactin, an antigen included in some acellular pertussis vaccines.

http://www.ncbi.nlm.nih.gov/pubmed/24355701