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© Research
Publication : Gut

USF1 Defect Drives p53 Degradation During Helicobacter pylori Infection and Accelerates Gastric Carcinogenesis

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Gut - 10 Dec 2019

Lionel Costa , Sébastien Corre , Valérie Michel , Krysten Le Luel , Julien Fernandes, Jason Ziveri , Gregory Jouvion, Anne Danckaert, Nicolas Mouchet, David Da Silva Barreira, Javier Torres, Margarita Camorlinga, Mario Milco D'Elios , Laurence Fiette , Hilde De Reuse, Marie-Dominique Galibert, Eliette Touati

Link to Pubmed [PMID] – 31822580

Link to DOI [DOI] – 10.1136/gutjnl-2019-318640.

Gut. 2019 Dec 10:gutjnl-2019-318640.

Abstract

Objective: Helicobacter pylori (Hp) is a major risk factor for gastric cancer (GC). Hp promotes DNA damage and proteasomal degradation of p53, the guardian of genome stability. Hp reduces the expression of the transcription factor USF1 shown to stabilise p53 in response to genotoxic stress. We investigated whether Hp-mediated USF1 deregulation impacts p53-response and consequently genetic instability. We also explored in vivo the role of USF1 in gastric carcinogenesis.

Design: Human gastric epithelial cell lines were infected with Hp7.13, exposed or not to a DNA-damaging agent camptothecin (CPT), to mimic a genetic instability context. We quantified the expression of USF1, p53 and their target genes, we determined their subcellular localisation by immunofluorescence and examined USF1/p53 interaction. Usf1 -/- and INS-GAS mice were used to strengthen the findings in vivo and patient data examined for clinical relevance.

Results: In vivo we revealed the dominant role of USF1 in protecting gastric cells against Hp-induced carcinogenesis and its impact on p53 levels. In vitro, Hp delocalises USF1 into foci close to cell membranes. Hp prevents USF1/p53 nuclear built up and relocates these complexes in the cytoplasm, thereby impairing their transcriptional function. Hp also inhibits CPT-induced USF1/p53 nuclear complexes, exacerbating CPT-dependent DNA damaging effects.

Conclusion: Our data reveal that the depletion of USF1 and its de-localisation in the vicinity of cell membranes are essential events associated to the genotoxic activity of Hp infection, thus promoting gastric carcinogenesis. These findings are also of clinical relevance, supporting USF1 expression as a potential marker of GC susceptibility.

Keywords: DNA damage; gastric cancer; genetic instability; helicobacter pylori infection; oncogenes.

http://dx.doi.org/10.1136/gutjnl-2019-318640