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© Research
Publication : Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Thiophilic adsorption revisited

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 01 Sep 2006

Hardouin J, Duchateau M, Canelle L, Vlieghe C, Joubert-Caron R, Caron M

Link to Pubmed [PMID] – 16949892

J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007 Jan;845(2):226-31

Specific and efficient selection of serum immunoglobulins, but not other proteins, on T-gel remains difficult. T-gel capacity was determined for different activation conditions and serum loadings. Mass spectrometry analysis was used to identify the proteins found in the flow-through and in the eluted fractions. Alpha-2-macroglobulin and albumin were the major contaminants of the eluates. The influence of the competition between immunoglobulins and the other serum proteins on the adsorption was also studied. Using a serum depleted in immunoglobulins (flow-through of a first chromatography on T-gel), many serum proteins were retained on the T-gel, including albumin. We conclude that T-gel selectivity is less than absolute and may reflect for a large part the experimental conditions of the adsorption.

http://www.ncbi.nlm.nih.gov/pubmed/16949892