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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Publication : European journal of biochemistry / FEBS

The structure of the O-glycosidic oligosaccharide chains of the major Zajdela hepatoma ascites-cell-membrane glycoprotein

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of biochemistry / FEBS - 01 Sep 1986

Nato F, Goulut C, Bourrillon R, Van Halbeek H, Vliegenthart JF

Link to Pubmed [PMID] – 3758066

Eur. J. Biochem. 1986 Sep;159(2):303-8

Glycoprotein MII2, the major cell surface glycoprotein (molecular mass 110 kDa) of Zajdela hepatoma ascites cells, contains about 25 O-glycosidic oligosaccharide chains per molecule. They were released as oligosaccharide-alditols by alkaline borohydride treatment of MII2, and purified by gel filtration on Bio-Gel P-6 followed by high-voltage paper electrophoresis. Four oligosaccharide-alditol fractions (A-D) were obtained in relative yields of 8:6:3:3. The structure of the components of fractions A-C was determined by 500-MHz 1H-NMR spectroscopy in combination with sugar composition analysis, to be as follows. (A) NeuAc alpha(2—-3)Gal beta(1—-3)[NeuAc alpha(2—-3)Gal beta(1—-4)GlcNAc beta(1—-6)]GalNAc-ol; (B1) NeuAc alpha(2—-3)Gal beta(1—-3)[Gal beta(1—-4)GlcNAc beta(1—-6)]GalNAc-ol; (B2) Gal beta(1—-3)[NeuAc alpha(2—-3)Gal beta(1—-4)GlcNAc beta(1—-6)]GalNAc-ol; (C) NeuAc alpha(2—-3)Gal beta(1—-3)GalNAc-ol. On the basis of sugar composition and characteristics on Bio-Gel P-6 filtration, paper electrophoresis and thin-layer chromatography, the structure of the carbohydrate component of fraction D is proposed to be as follows. (D) NeuAc alpha(2—-3)Gal beta(1—-3)[NeuAc alpha(2—-6)]GalNAc-ol

http://www.ncbi.nlm.nih.gov/pubmed/3758066