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© Research
Publication : Methods Mol Biol.

Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast (Book chapter).

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods Mol Biol. - 01 Jan 2016

Peña-Castillo L, Badis G.

Link to Pubmed [PMID] – 26483024

Methods Mol Biol. 2016;1361:203-25. doi: 10.1007/978-1-4939-3079-1_12.

Understanding how genes are regulated, decoding their “regulome”, is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.