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© Research
Publication : Nature

Structure of the outer membrane complex of a type IV secretion system

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature - 29 Nov 2009

Chandran V, Fronzes R, Duquerroy S, Cronin N, Navaza J, Waksman G

Link to Pubmed [PMID] – 19946264

Nature 2009 Dec;462(7276):1011-5

Type IV secretion systems are secretion nanomachines spanning the two membranes of Gram-negative bacteria. Three proteins, VirB7, VirB9 and VirB10, assemble into a 1.05 megadalton (MDa) core spanning the inner and outer membranes. This core consists of 14 copies of each of the proteins and forms two layers, the I and O layers, inserting in the inner and outer membrane, respectively. Here we present the crystal structure of a approximately 0.6 MDa outer-membrane complex containing the entire O layer. This structure is the largest determined for an outer-membrane channel and is unprecedented in being composed of three proteins. Unexpectedly, this structure identifies VirB10 as the outer-membrane channel with a unique hydrophobic double-helical transmembrane region. This structure establishes VirB10 as the only known protein crossing both membranes of Gram-negative bacteria. Comparison of the cryo-electron microscopy (cryo-EM) and crystallographic structures points to conformational changes regulating channel opening and closing.

http://www.ncbi.nlm.nih.gov/pubmed/19946264