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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Transactions of the Royal Society of Tropical Medicine and Hygiene

Staphylococci adherence to trypanosomes exposed to immune sera as a method for the diagnosis of Chagas’ disease

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Transactions of the Royal Society of Tropical Medicine and Hygiene - 01 Jan 1983

Camargo EP, Mattei DM, Yoshida N, Caulada Z

Link to Pubmed [PMID] – 6420951

Trans. R. Soc. Trop. Med. Hyg. 1983;77(6):825-8

A new method, the staphylococci adherence test (SAT), for the serological diagnosis of Chagas’ disease is described; it is based on the affinity of staphylococcal Protein A for IgG globulins and uses epimastigotes of Trypanosoma cruzi which are fixed on to glass slides and incubated stepwise with probing sera and a Staphylococcus aureus A suspension. After Giemsa staining, epimastigote preparations which have been incubated with positive sera appear covered by cocci. Results using indirect immunofluorescence, complement fixation and SAT were in agreement in 98.37% of 860 human sera. A slightly modified SAT (SAT’) may also be utilized for the diagnosis of acute Chagas’ disease. The simplicity of the method may allow its adaptation for field work. Other possible uses were investigated.

http://www.ncbi.nlm.nih.gov/pubmed/6420951