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© Institut Pasteur
Cryptococcus neoformans
Publication : FEMS microbiology letters

Selection and study of a Candida molischiana mutant derepressed for beta-glucosidase production

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEMS microbiology letters - 15 May 1994

Janbon G, Arnaud A, Galzy P

Link to Pubmed [PMID] – 8020743

FEMS Microbiol. Lett. 1994 May;118(3):207-11

A mutant strain of Candida molischiana was selected. Analysis of the exocellular activity of Candida molischiana 35M5N grown on different carbon sources revealed that the biosynthesis of beta-glucosidase is derepressed in this yeast strain. The strain is not a hyper-producer mutant. There were no observed differences in the endocellular and parietal activities of the wild and mutant strains. However, the mutant strain produced 35-fold more enzyme than the wild-type in the culture medium with glucose as carbon source. When glucose was used as carbon source, the mutant strain produced 90% more exocellular enzyme than when cellobiose was used as the carbon source.

http://www.ncbi.nlm.nih.gov/pubmed/8020743