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© Marie-Christine Prévost, Anne Derbise
Bactéries Yersinia pestis en microscopie electronique à balayage.
Publication : Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Reassessing the role of Internalin B in Listeria monocytogenes virulence using the epidemic strain F2365

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases - 08 Jul 2019

Quereda JJ, Rodríguez-Gómez IM, Meza-Torres J, Gómez-Laguna J, Nahori MA, Dussurget O, Carrasco L, Cossart P, Pizarro-Cerdá J

Link to Pubmed [PMID] – 30195066

Clin. Microbiol. Infect.

OBJECTIVES: We investigated the contribution to virulence of the surface protein Internalin B (InlB) in the L. monocytogenes lineage I strain F2365, which caused in California in 1985 one of the deadliest listeriosis outbreaks.

METHODS: The F2365 strain displays a point mutation that hampers expression of InlB. We rescued the expression of InlB in the L. monocytogenes lineage I strain F2365 introducing a point mutation in the codon 34 (TAA to CAA) and investigated its importance for bacterial virulence using in vitro cell infection systems and a murine intravenous infection model.

RESULTS: We show in HeLa and JEG-3 cells that the F2365 InlB+ strain expressing InlB was ≈9-fold and ≈1.5-fold more invasive than F2365, respectively. In livers and spleens of infected mice at 72 h post-infection bacterial counts for F2365 InlB+ were significantly higher compared to the F2365 strain (≈1 log more) and histopathological assessment showed that the F2365 strain displayed a reduced number of necrotic foci compared to the F2365 InlB+ strain (Mann-Whitney test).

CONCLUSIONS: We describe a critical role for InlB during infection of non-pregnant animals by a L. monocytogenes strain from lineage I. Our study suggests that a spontaneous mutation in InlB could have prevented more severe human morbidity and mortality during the 1985 California listeriosis outbreak.

https://www.ncbi.nlm.nih.gov/pubmed/30195066