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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Protein sample characterization

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2013

Daviter T, Fronzes R

Link to Pubmed [PMID] – 23729248

Methods Mol. Biol. 2013;1008:35-62

Most biophysical experiments require protein samples of high quality and accurately determined concentration. This chapter attempts to compile basic information on the most common methods to assess the purity, dispersity, and stability of protein samples. It also reminds of methods to measure protein concentration and of their limits. The idea is to make aware and remind of the range of methods available and of commonly overlooked pitfalls. The aim is to enable experimenters to fully characterize their preparations of soluble or membrane proteins and gain reliable and reproducible results from their experimental work.

http://www.ncbi.nlm.nih.gov/pubmed/23729248