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© A. Alanio, E. Perret
Prolifération de Cryptococcus neoformans dans des macrophages murins.
Publication : Diagnostic microbiology and infectious disease

Pneumocystis jirovecii in the air surrounding patients with Pneumocystis pulmonary colonization

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Diagnostic microbiology and infectious disease - 15 Jan 2015

Le Gal S, Pougnet L, Damiani C, Fréalle E, Guéguen P, Virmaux M, Ansart S, Jaffuel S, Couturaud F, Delluc A, Tonnelier JM, Castellant P, Le Meur Y, Le Floch G, Totet A, Menotti J, Nevez G

Link to Pubmed [PMID] – 25801779

Diagn. Microbiol. Infect. Dis. 2015 Jun;82(2):137-42

In this study, Pneumocystis jirovecii was detected and characterized in the air surrounding patients with Pneumocystis pulmonary colonization. Air samples were collected in the rooms of 10 colonized patients using Coriolis® μ air sampler at 1m and 5m from the patient’s head. P. jirovecii DNA was amplified and genotyped in pulmonary and air samples at the mitochondrial large subunit ribosomal RNA gene. P. jirovecii DNA was detected in 5 of the 10 air samples collected at 1m and in 5 of the 10 other air samples collected at 5m. P. jirovecii genotyping was successful in 4 pairs or triplets of air and pulmonary samples. Full genotype matches were observed in 3 of the 4 pairs or triplets of air and pulmonary samples. These results provide original data supporting P. jirovecii exhalation from colonized patients and emphasize the risk of P. jirovecii nosocomial transmission from this patient population.

http://www.ncbi.nlm.nih.gov/pubmed/25801779