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© Research
Publication : Journal of virology

ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 03 Mar 2010

Goulet A, Pina M, Redder P, Prangishvili D, Vera L, Lichière J, Leulliot N, van Tilbeurgh H, Ortiz-Lombardia M, Campanacci V, Cambillau C

Link to Pubmed [PMID] – 20200253

J. Virol. 2010 May;84(10):5025-31

Acidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. It infects Acidianus species that thrive in the acidic hot springs (>85 degrees C and pH <3) of Yellowstone National Park, WY. The AFV1 20.8-kb, linear, double-stranded DNA genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. Because three-dimensional structures are more conserved than sequences and hence are more effective at revealing function, we set out to determine protein structures from putative AFV1 open reading frames (ORF). The crystal structure of ORF157 reveals an alpha+beta protein with a novel fold that remotely resembles the nucleotidyltransferase topology. In vitro, AFV1-157 displays a nuclease activity on linear double-stranded DNA. Alanine substitution mutations demonstrated that E86 is essential to catalysis. AFV1-157 represents a novel class of nuclease, but its exact role in vivo remains to be determined.

http://www.ncbi.nlm.nih.gov/pubmed/20200253