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© Research
Publication : Journal of microbiological methods

On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of microbiological methods - 16 Jan 2007

Daugelavicius R, Gaidelyte A, Cvirkaite-Krupovic V, Bamford DH

Link to Pubmed [PMID] – 17289190

J. Microbiol. Methods 2007 Apr;69(1):174-9

In this study an on-line electrochemical method was developed to examine the one-step growth cycle (OSGC) of the bacteriophage Bam35. The on-line conditions for monitoring the OSGC and the effect of aeration on the duration of the OSGC were defined. The data indicate that binding of phenyldicarbaundecaborane anions to Bacillus thuringiensis cells infected with Bam35 can be used as a sensitive indicator of cell lysis.

http://www.ncbi.nlm.nih.gov/pubmed/17289190