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© Research
Publication : Molecular and cellular probes

Non-radioactive gene probes for the detection of tetracycline and/or minocycline resistance in staphylococci

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular and cellular probes - 01 Dec 1988

Sakamoto H, Zilhao R, Bismuth R, Courvalin P, Guesdon JL

Link to Pubmed [PMID] – 3073314

Mol. Cell. Probes 1988 Dec;2(4):321-30

This report describes the development of a non-radioactive gene probe for the characterization of tetracycline and/or minocycline resistant staphylococci. Both radio-labelled and non-radioactive gene probes yielded a better characterization of the strains than the determination of minimal inhibitory concentration (MIC). A 100% correlation was found between radioactive and non-radioactive hybridization tests. The latter technique detected genes present in a single copy per bacterial genome, could be performed in 8 h, avoided radioactive hazards and autoradiography delay, and appeared therefore to be a useful tool for clinical and epidemiological studies.

http://www.ncbi.nlm.nih.gov/pubmed/3073314