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© Research
Publication : European journal of biochemistry / FEBS

Molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type 1a DNA

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of biochemistry / FEBS - 01 Aug 1980

Danos O, Katinka M, Yaniv M

Link to Pubmed [PMID] – 6250842

Eur. J. Biochem. 1980 Aug;109(2):457-61

The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. The integrity and the homogeneity of the viral DNA thus obtained was confirmed by restriction endonucleases analysis. Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome.

http://www.ncbi.nlm.nih.gov/pubmed/6250842