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© Research
Publication : Epidemiology and infection

Molecular characterization of Leptospira spp. strains isolated from small rodents in Croatia

Member: Natacha Sertour
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Epidemiology and infection - 01 Feb 2003

Turk N, Milas Z, Margaletic J, Staresina V, Slavica A, Riquelme-Sertour N, Bellenger E, Baranton G, Postic D

Link to Pubmed [PMID] – 12613757

Epidemiol. Infect. 2003 Feb;130(1):159-66

We report the isolation and characterization of 16 Leptospira spp. strains isolated from small rodents captured in 11 different regions of inland Croatia. Large NotI and SgrAI restriction fragment allowed us to assign 10 isolates to the serovar istrica, 5 isolates to the serovar tsaratsovo and 1 isolate to the serovar lora. The phylogenetic analysis conducted from the sequences of the first 330 bp from the 16S rDNA gene revealed that the strains belonged to three different species, L. borgpetersenii, L. kirschneri and L. interrogans. Carrier rates in eight rodent species varied from 0 to 71.4%. Mus musculus showed the highest infection level and confirmed its role as a major reservoir of the serogroup Sejroë. For the first time we reported the occurrence of serovars tsaratsovo and lora in Croatia.