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© Research
Publication : Bio-protocol

Measurement of Dipeptidylpeptidase Activity in vitro and in vivo.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Bio-protocol - 20 Mar 2017

da Silva RB, Ingersoll MA, Albert ML,

Link to Pubmed [PMID] – 34458493

Link to DOI – 10.21769/BioProtoc.2184

Bio Protoc 2017 Mar; 7(6): e2184

Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space. DPP4 is found at the surface of many hematopoietic and non-hematopoietic cells and is also present in many biological fluids in a bioactive soluble form. DPP4 expression is modulated by inflammation, and measurements of its activity have been used as biomarker for disease. Here, we describe a method to evaluate the enzymatic activity of DPP4 in vitro and in vivo.

https://pubmed.ncbi.nlm.nih.gov/34458493