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Enzymatic synthesis of guanine nucleotides labeled with 15N at the 2-amino group of the purine ring

The bovine papillomavirus 1 E2 protein contains two activation domains: one that interacts with TBP and another that functions after TBP binding

© Artur Scherf

Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.

Publication : Infection and immunity

Karyotype analysis of virulent Plasmodium falciparum strains propagated in Saimiri sciureus: strain adaptation leads to deletion of the RESA gene

Team: Biology of Host-parasite Interactions

Member: Denise Mattei

Scientific Fields

Diseases

Organisms

Applications

Technique

Published in Infection and immunity - 01 Feb 1995

Hinterberg K, Muanza K, Hernandez-Rivas R, Gay F, Gysin J, Mattei D, Scherf A

Link to Pubmed [PMID] – 7822042

Infect. Immun. 1995 Feb;63(2):693-5

The squirrel monkey, Saimiri sciureus, is an important experimental model for the study of the human malaria parasite Plasmodium falciparum. A detailed karyotype analysis of four different P. falciparum strains propagated in S. sciureus was done using various subtelomeric antigen gene probes. We observed deletion of the complete RESA gene from chromosome 1 in all four strains. Interestingly, a loss of RESA was observed immediately upon adaptation to the squirrel monkey, suggesting that this DNA rearrangement is fundamental for the P. falciparum infection of S. sciureus erythrocytes. However, a RESA-specific gene probe hybridized with chromosome 1 of wild isolates from 28 different patients, indicating that this gene is maintained during infection of humans.

Published on: 01 Feb 1995