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© Marie-Christine Prévost, Anne Derbise
Bactéries Yersinia pestis en microscopie electronique à balayage.
Publication : Methods in molecular biology (Clifton, N.J.)

Internalization Assays for Listeria monocytogenes.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2021

Kühbacher A, Cossart P, Pizarro-Cerdá J,

Link to Pubmed [PMID] – 32975776

Link to DOI [DOI] – 10.1007/978-1-0716-0982-8_15

Methods Mol. Biol. 2021 ; 2220: 189-200

Listeria monocytogenes is a model intracellular pathogen that can invade the cytoplasm of host mammalian cells. Cellular invasion can be measured using standard techniques, such as the classical gentamicin protection assay, based on the quantification of colony-forming units from lysates of infected cells. In addition, there are methods based on immunofluorescence microscopy which allow for assaying invasion in a medium- to high-throughput manner. In the following sections, we detail two different assays that can be used alone or in combination to quantify the internalization of L. monocytogenes in host cells.

https://pubmed.ncbi.nlm.nih.gov/32975776