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© Melanie Blokesch, EPFL
Flagellated Vibrio cholerae
Publication : Methods in molecular biology (Clifton, N.J.)

Integron Identification in Bacterial Genomes and Cassette Recombination Assays.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2020

Vit C, Loot C, Escudero JA, Nivina A, Mazel D,

Link to Pubmed [PMID] – 31584164

Link to DOI – 10.1007/978-1-4939-9877-7_14

Methods Mol Biol 2020 ; 2075(): 189-208

Integrons are genetic elements involved in bacterial adaptation to the environment. Sedentary chromosomal integrons (SCIs) can stockpile and rearrange a myriad of different functions encoded in gene cassettes. Through their association with transposable elements and conjugative plasmids, some SCIs have acquired mobility and are now termed Mobile Integrons (MIs). MIs have reached the hospitals and are involved in the rise and spread of antibiotic resistance genes through horizontal gene transfer among numerous bacterial species. Here we aimed at describing methods for the detection of integrons in sequenced bacterial genomes as well as for the experimental characterization of the activity of their different components: the integrase and the recombination sites.

https://pubmed.ncbi.nlm.nih.gov/31584164