doi: 10.1186/1756-3305-5-264. PMID: 23153178 Free PMC Article Similar articles << First< PrevPage of 3Next >Last >> Back to top Supplemental Content Filters: Manage Filters Sort by: Best matchMost recent Find related data Database: Search details choumet[All Fields] See more... Recent Activity Clear Turn Off Infection by chikungunya virus modulates the expression of several proteins in A... PubMed Visualizing non infectious and infectious Anopheles gambiae blood feedings in na... PubMed New markers in Anopheles gambiae salivary glands after Plasmodium berghei infect... PubMed Aedes mosquito saliva modulates Rift Valley fever virus pathogenicity. PubMed Differential expression of Ixodes ricinus salivary gland proteins in the presenc... PubMed See more... You are here: NCBI > Literature > PubMed Support Center
Arthropod-borne viral infections cause several emerging and resurging infectious diseases. Among the diseases caused by arboviruses, chikungunya is responsible for a high level of severe human disease worldwide. The salivary glands of mosquitoes are the last barrier before pathogen transmission.
We undertook a proteomic approach to characterize the key virus/vector interactions and host protein modifications that occur in the salivary glands that could be responsible for viral transmission by using quantitative two-dimensional electrophoresis.
We defined the protein modulations in the salivary glands of Aedes aegypti that were triggered 3 and 5 days after an oral infection (3 and 5 DPI) with chikungunya virus (CHIKV). Gel profile comparisons showed that CHIKV at 3 DPI modulated the level of 13 proteins, and at 5 DPI 20 proteins. The amount of 10 putatively secreted proteins was regulated at both time points. These proteins were implicated in blood-feeding or in immunity, but many have no known function. CHIKV also modulated the quantity of proteins involved in several metabolic pathways and in cell signalling.
Our study constitutes the first analysis of the protein response of Aedes aegypti salivary glands infected with CHIKV. We found that the differentially regulated proteins in response to viral infection include structural proteins and enzymes for several metabolic pathways. Some may favour virus survival, replication and transmission, suggesting a subversion of the insect cell metabolism by arboviruses. For example, proteins involved in blood-feeding such as the short D7, an adenosine deaminase and inosine-uridine preferring nucleoside hydrolase, may favour virus transmission by exerting an increased anti-inflammatory effect. This would allow the vector to bite without the bite being detected. Other proteins, like the anti-freeze protein, may support vector protection.