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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Applied and environmental microbiology

Identification of Listeria monocytogenes genes involved in salt and alkaline-pH tolerance

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied and environmental microbiology - 01 Jun 2003

Gardan R, Cossart P, Labadie J,

Link to Pubmed [PMID] – 12788708

Appl. Environ. Microbiol. 2003 Jun;69(6):3137-43

The capacity of Listeria monocytogenes to tolerate salt and alkaline stresses is of particular importance, as this pathogen is often exposed to such environments during food processing and food preservation. We screened a library of Tn917-lacZ insertional mutants in order to identify genes involved in salt and/or alkaline tolerance. We isolated six mutants sensitive to salt stress and 12 mutants sensitive to salt and alkaline stresses. The position of the insertion of the transposon was located in 15 of these mutants. In six mutants the transposon was inserted in intergenic regions, and in nine mutants it was inserted in genes. Most of the genes have unknown functions, but sequence comparisons indicated that they encode putative transporters.