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© Research
Publication : Iatreia

Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Iatreia - 01 Jul 2014

Félix Giovanni Delgado Tiria, Adriana Marisol Pérez Acosta, Jaime E. Castellanos

IATREIA. 2014; 27(3): 267-276

To date, there are no appropriate animal models for the study of the pathophysiology and clinical manifestations of the disease caused by dengue virus infection; therefore, experimental models are required for that purpose. The objective of the present work was to establish a model of in vitro infection with DENV-2. To this end, human peripheral blood mononuclear cells (PBMC) were obtained using a Ficoll gradient, and infected with DENV-2 using a low multiplicity of infection. The cell populations infected and responsible for the production of cytokines were identified using a multiparametric analysis by flow cytometry. As a result, PBMC were permissive to infection that was detected 24 hours after virus inoculation. Additionally, at this same time, CD14+ cells, but not CD3+ or CD19+ cells, were preferentially infected and responsible for the production of TNF-α and IL-6. In conclusion, we established a model of in vitro infection using unfractionated PBMC, in which CD14+ cells were identified as the primary target cells for infection with DENV-2, and the production of proinflammatory cytokines.

http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/16221/16762