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© Institut Pasteur
Cristaux de cellulase, enzyme purifiée de Clostridium thermocellum permettant la digestion de la cellulose. Image colorisée.
Publication : Research in microbiology

How to broaden enzyme substrate specificity: strategies, implications and applications

Team: Group : Chemistry and Biocatalysis
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Research in microbiology - 18 Oct 2005

Jestin JL, Vichier-Guerre S

Link to Pubmed [PMID] – 16293399

Res. Microbiol. 2005 Dec;156(10):961-6

For identification of mutations associated with the broadening of enzyme substrate specificity, three strategies, including directed enzyme evolution, are described for selected examples. Implications concerning enzyme models are highlighted. Applications to the field of biocatalysis are discussed. A bidimensional map for the classification of enzyme activities is suggested so as to improve genome annotations.