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© Research
Publication : Biotechnology and bioengineering

Factors controlling cell proliferation and antibody production in mouse hybridoma cells: I. Influence of the amino acid supply

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biotechnology and bioengineering - 01 Sep 1991

Duval D, Demangel C, Munier-Jolain K, Miossec S, Geahel I

Link to Pubmed [PMID] – 18604875

Biotechnol. Bioeng. 1991 Sep;38(6):561-70

To investigate the influence of medium amino acid resources on hybridoma cell proliferation and monoclonal antibody secretion, we first determined, in two different cell lines, the pattern of amino acid consumption. We then showed that complementation of each cell line by an amino acid solution prepared to cover its needs led in both cases to a marked increase in cell density and monoclonal antibody production. This observation suggests that the amino acid supply is one of the factors limiting cell growth and productivity in typical batch processes, and we demonstrated that a similar limitation occurs in a semicontinuous culture. Finally, we showed that the cell decline observed after a period of amino acid deficiency is not irreversible and that proliferation could be resumed if the required amino acids were added.

http://www.ncbi.nlm.nih.gov/pubmed/18604875