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© Research
Publication : Journal of clinical microbiology

Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates

Team: Archived: Molecular Prevention and Therapy of Human Diseases
Member: Sophie Guillot
Member: Nicole Guiso
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of clinical microbiology - 14 Sep 2011

Lanotte P, Plouzeau C, Burucoa C, Grélaud C, Guillot S, Guiso N, Garnier F

Link to Pubmed [PMID] – 21918018

J. Clin. Microbiol. 2011 Nov;49(11):3943-6

We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.