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© Research
Publication : Bioorganic and Medicinal Chemistry

Enzymatic synthesis of biphenyl-DNA oligonucleotides

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Bioorganic and Medicinal Chemistry - 07 May 2020

Pascal Röthlisberger, Fabienne Levi-Acobas, Christian J. Leumann, Marcel Hollenstein*

Biorg. Med. Chem. 2020, 28, 115487

The incorporation of nucleotides equipped with C-glycosidic aromatic nucleobases into DNA and RNA is an alluring strategy for a number of practical applications including fluorescent labelling of oligonucleotides, expansion of the genetic alphabet for the generation of aptamers and semi-synthetic organisms, or the modulation of excess electron transfer within DNA. However, the generation of C-nucleoside containing oligonucleotides relies mainly on solid-phase synthesis which is quite labor intensive and restricted to short sequences. Here, we explore the possibility of constructing biphenyl-modified DNA sequences using enzymatic synthesis. The presence of multiple biphenyl-units or biphenyl residues modified with electron donors and acceptors permits the incorporation of a single dBphMP nucleotide. Moreover, templates with multiple abasic sites enable the incorporation of up to two dBphMP nucleotides, while TdT-mediated tailing reactions produce single-stranded DNA oligonucleotides with four biphenyl residues appended at the 3′-end.

https://www.sciencedirect.com/science/article/pii/S0968089620303084