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© Research
Publication : Diagnostic microbiology and infectious disease

Development of real-time PCR assay for differential detection of Bordetella bronchiseptica and Bordetella parapertussis

Team: Archived: Molecular Prevention and Therapy of Human Diseases
Member: Sophie Guillot
Member: Nicole Guiso
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Diagnostic microbiology and infectious disease - 14 Jan 2014

Tizolova A, Brun D, Guiso N, Guillot S

Link to Pubmed [PMID] – 24525142

Diagn. Microbiol. Infect. Dis. 2014 Apr;78(4):347-51

Bordetella parapertussis is a causative agent of whooping cough in humans, and B. bronchiseptica is causing wide variety of respiratory infections in mammals, including humans. Specific diagnostic tests are not currently available. Our first objective was to develop a real-time PCR test for the specific detection of B. bronchiseptica based on the previously described end-point PCR, targeting an intergenomic sequence of the fla gene locus, but it has not been reached. However, there is cross-reactivity between B. parapertussis and B. bronchiseptica. Therefore, the targeted region of several clinical isolates of both species was sequenced, and alignment of the sequences allowed the development of a 2-step real-time PCR assay. The first PCR assay detected the DNA of all clinical isolates of both B. bronchiseptica and B. parapertussis tested. The second PCR assay detected only the DNA of B. parapertussis clinical isolates, thereby allowing discrimination between B. parapertussis and B. bronchiseptica.

http://www.ncbi.nlm.nih.gov/pubmed/24525142