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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Publication : Journal of immunological methods

Development of a bispecific monoclonal antibody for use in molecular hybridisation

Team: Antibody Engineering
Member: Farida Nato
Member: Pierre Lafaye
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of immunological methods - 28 Feb 1994

Auriol J, Guesdon JL, Mazié JC, Nato F

Link to Pubmed [PMID] – 8133070

J. Immunol. Methods 1994 Feb;169(1):123-33

A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.

http://www.ncbi.nlm.nih.gov/pubmed/8133070