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© Research
Publication : European journal of immunology

Default development of cloned human naive CD4 T cells into interleukin-4- and interleukin-5- producing effector cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of immunology - 01 Dec 1995

Yang LP, Byun DG, Demeure CE, Vezzio N, Delespesse G

Link to Pubmed [PMID] – 8566047

Eur. J. Immunol. 1995 Dec;25(12):3517-20

It was recently demonstrated that naive human and mouse CD4 T cells release low but sufficient levels of interleukin (IL)-4 at priming to support their development into IL-4 producers. To determine whether this IL-4 is produced by a minor subset of cells, freshly isolated human naive CD4 T cells were directly cloned by limiting dilution in the absence of exogenous IL-4. More than 95% of neonatal and 60% of adult naive T cells seeded in single-cell cultures could be expanded upon stimulation with anti-CD3 mAb immobilized on CD32-B7.1 L cell transfectants in the presence of IL-2. All 171 clones derived from four neonates and two adults produced IL-4 and IL-5 at generally high levels. Like the allergen-specific human Th2 clones described in the literature, these T cell clones produced little or no interferon-gamma upon stimulation via their T cell receptor/CD3 complex, whereas they released high levels of this cytokine when activated with phorbol 12-myristate 13-acetate+ionomycin. Cells cloned and expanded in the presence of anti-IL4 + anti-IL-4R mAb produced much lower levels of IL-4 and IL-5. It is concluded that almost every single naive human CD4 T cell primed and expanded in the absence of exogenous IL-4 releases sufficient autocrine IL-4 to support its clonal expansion into high IL-4/IL-5 producers.

http://www.ncbi.nlm.nih.gov/pubmed/8566047