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© Research
Publication : Imaging and Microscopy

Cryo-electron Tomography of Whole Cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Imaging and Microscopy - 15 Sep 2007

Cyrklaff M, Chlanda P, Sartori A, Lepper S

Imaging and Microscopy 2007 9:50-53

Cryo-electron tomography was used for studying the molecular organization of the cytoplasm in intact cells. Flat areas of cells grown on EM grids and preserved by rapid freezing within milliseconds were imaged in 3D. In the resulting maps the selected morphological and structural features were analyzed at unprecedented detail, and frequently correlated to light-microscopy images. We portray here host cell-virus interactions, the morphology of neurons and malaria parasites.

https://analyticalscience.wiley.com/do/10.1002/imaging.582/full/