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© Institut Pasteur
Cryptococcus neoformans
Publication : Gene

Cloning and sequencing of the beta-glucosidase-encoding gene from Candida molischiana strain 35M5N

Team: RNA Biology of Fungal Pathogens
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Gene - 01 Nov 1995

Janbon G, Magnet R, Arnaud A, Galzy P

Link to Pubmed [PMID] – 7489898

Gene 1995 Nov;165(1):109-13

We have isolated a gene (bgln) encoding beta-glucosidase (beta Glu) from a cosmid library of the yeast, Candida molischiana 35M5N. The nucleotide sequence of bgln and its flanking regions was determined. This gene was found to be composed of 2289 bp and 763 amino acid (aa) residues encoding an 83.3-kDa protein. The aa sequence shared eleven putative N-glycosylation sites. Homology comparisons showed that this enzyme can be considered as a new member of the family-3 glycosyl hydrolases. Multiple alignment experiments revealed four conserved regions on aa sequences from beta Glu of this family.