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© Andreas Kühbacher, Edith Gouin, Jason Mercer, Mario Emmenlauer, Christophe Dehio, Pascale Cossart and Javier Pizarro-Cerda
Immunofluorescence and segmentation analysis of HeLa cells infected with Listeria monocytogenes. Cells were labelled with DAPI to mark DNA (blue), with phalloidin to mark actin (red) and with anti-Internalin C antibodies to identify infected cells (yellow). Nuclei and cytoplasms were segmented using the public image analysis software CellProfiler
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2017

Quereda JJ, Sachse M, Balestrino D, Grenier T, Fredlund J, Danckaert A, Aulner N, Shorte S, Enninga J, Cossart P, Pizarro-Cerdá J

Link to Pubmed [PMID] – 27914079

Methods Mol. Biol. 2017;1535:173-195

Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.

https://www.ncbi.nlm.nih.gov/pubmed/27914079