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Scientific Fields
Diseases
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Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2017

Quereda JJ, Sachse M, Balestrino D, Grenier T, Fredlund J, Danckaert A, Aulner N, Shorte S, Enninga J, Cossart P, Pizarro-Cerdá J

Link to Pubmed [PMID] – 27914079

Methods Mol. Biol. 2017;1535:173-195

Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.

https://www.ncbi.nlm.nih.gov/pubmed/27914079