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© Research
Publication : The Journal of biological chemistry

Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of biological chemistry - 15 Jul 1994

Bischof O, Kruft V, Wittmann-Liebold B

Link to Pubmed [PMID] – 8034577

J. Biol. Chem. 1994 Jul;269(28):18315-9

Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following peptides were found labeled: Pro1-Lys10 of S7, Ala28-Lys46 and Ala7-Lys11 of S14, Asp24-Lys29 of S18, Tyr64-Lys68 of L18, and Thr55-Lys60 of L29. For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level.

http://www.ncbi.nlm.nih.gov/pubmed/8034577