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Assistant Professor
Associate Professor
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Clinical Research Nurse
Clinician Researcher
Department Manager
Dual-education Student
Full Professor
Honorary Professor
Lab assistant
Master Student
MD-PhD Student
Medical Staff
Non-permanent Researcher
Nursing Staff
Permanent Researcher
Pharmacist
PhD Student
Physician
Post-doc
Prize
Project Manager
Research Associate
Research Engineer
Retired scientist
Technician
Undergraduate Student
Veterinary
Visiting Scientist

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Deputy Director of Center
Deputy Director of Department
Deputy Director of National Reference Center
Deputy Head of Facility
Director of Center
Director of Department
Director of Institute
Director of National Reference Center
Group Leader
Head of Facility
Head of Operations
Head of Structure
Honorary President of the Departement
Labex Coordinator

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Global Organization and Proposed Megataxonomy of the Virus World

Active diffusion in oocytes nonspecifically centers large objects during prophase I and meiosis I.

Publication : Genome biology

Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS.

Team: Microbial Paleogenomics Unit

Member: Maria Lopopolo

Scientific Fields

Diseases

Organisms

Applications

Technique

Published in Genome biology - 03 Mar 2020

Liu Y, Cheng J, Siejka-Zielińska P, Weldon C, Roberts H, Lopopolo M, Magri A, D'Arienzo V, Harris JM, McKeating JA, Song CX

Link to Pubmed [PMID] – 32127008

Link to DOI – 10.1186/s13059-020-01969-6

Genome Biol 2020 Mar; 21(1): 54

We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.

Published on: 03 Mar 2020